{"_buckets": {"deposit": "87b16d9d-3744-4fc9-943d-fc65bd403ecf"}, "_deposit": {"created_by": 15, "id": "15320", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "15320"}, "status": "published"}, "_oai": {"id": "oai:mie-u.repo.nii.ac.jp:00015320", "sets": ["1692755240724"]}, "author_link": ["50753", "50752", "50751"], "item_8_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2021-05-17", "bibliographicIssueDateType": "Issued"}}]}, "item_8_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kimura, Tetsuya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "50753", "nameIdentifierScheme": "WEKO"}]}]}, "item_8_description_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_8_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "海洋性バイオマスのキチンを効率的に分解し水素ガスを高生産する嫌気性細菌 Clostridium paraputrificumを利用して、環境に負荷をかけることなくバイオマスを分解し、水素エネルギーとして回収することを究極の目的として、本菌の水素ガス生産経路の解明を分子生物学的な遺伝子破壊方法を応用して解析した。その結果、ピルビン酸からアセチルCoAへの変換経路が水素ガス生産に重要であり、特に嫌気性細菌特有の酵素であるピルビン酸フェレドキシンオキシドレダクターゼは生育に必須であること、乳酸生産からアセチルCoA生産へ代謝の流れが多くなることで水素ガス生産が増加することが明らかとなった。", "subitem_description_type": "Abstract"}, {"subitem_description": "The ultimate goal is to use the anaerobic bacterium Clostridium paraputrificum to produce bio-hydrogen gas, which efficiently use the marine biomass chitin as an energy source without the environment load. The hydrogen gas production pathway of this bacterium was analyzed by applying the recent molecular biological gene disruption method, ClosTron technology. As a result, the conversion pathway from pyruvate to acetyl-CoA is important for hydrogen gas production, and pyruvate ferredoxin oxidoreductase, which catalyzes this step peculiar to anaerobic bacteria, is essential for growth. It was clarified that inhibition of lactate production pathway increased hydrogen gas production due to the metabolic flow change. When pyruvate to lactate is blocked, flow from pyruvate to acetyl-CoA by pyruvate ferredoxin oxidoreductase would be increased.", "subitem_description_type": "Abstract"}]}, "item_8_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "2018年度～2020年度科学研究費補助金(基盤研究(C))研究成果報告書", "subitem_description_type": "Other"}]}, "item_8_description_64": {"attribute_name": "科研費番号", "attribute_value_mlt": [{"subitem_description": "18K11708", "subitem_description_type": "Other"}]}, "item_8_full_name_2": {"attribute_name": "著者（ヨミ）", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "50752", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "キムラ, テツヤ", "nameLang": "ja-Kana"}]}]}, "item_8_publisher_30": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "三重大学"}]}, "item_8_text_31": {"attribute_name": "出版者（ヨミ）", "attribute_value_mlt": [{"subitem_text_value": "ミエダイガク"}]}, "item_8_text_65": {"attribute_name": "資源タイプ（三重大）", "attribute_value_mlt": [{"subitem_text_value": "Kaken / 科研費報告書"}]}, "item_8_version_type_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "木村, 哲哉", "creatorNameLang": "ja"}], "nameIdentifiers": [{"nameIdentifier": "50751", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-11-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2022RP0081.pdf", "filesize": [{"value": "153.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 153600.0, "url": {"label": "2022RP0081", "url": "https://mie-u.repo.nii.ac.jp/record/15320/files/2022RP0081.pdf"}, "version_id": "e48cad2d-23d8-4211-ba59-1a764cbab5f8"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Clostridium", "subitem_subject_scheme": "Other"}, {"subitem_subject": "水素ガス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "バイオマス", "subitem_subject_scheme": "Other"}, {"subitem_subject": "遺伝子工学", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "バイオ水素ガス生産の基盤技術となる嫌気性細菌の遺伝子改変技術確立とその応用", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "バイオ水素ガス生産の基盤技術となる嫌気性細菌の遺伝子改変技術確立とその応用", "subitem_title_language": "ja"}, {"subitem_title": "Development of gene engineering method for anaerobic bacteria for efficient bio-hydrogen production", "subitem_title_language": "en"}]}, "item_type_id": "8", "owner": "15", "path": ["1692755240724"], "permalink_uri": "http://hdl.handle.net/10076/00020938", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-11-29"}, "publish_date": "2022-11-29", "publish_status": "0", "recid": "15320", "relation": {}, "relation_version_is_last": true, "title": ["バイオ水素ガス生産の基盤技術となる嫌気性細菌の遺伝子改変技術確立とその応用"], "weko_shared_id": -1}