WEKO3
アイテム
Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulumstress through SEL1L upregulation
http://hdl.handle.net/10076/00021051
http://hdl.handle.net/10076/00021051ffc7c736-31cf-4037-92ed-ac00efd6d728
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2022DM0908 (1.6 MB)
|
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-28 | |||||||
| タイトル | ||||||||
| タイトル | Suppression of RNF213, a susceptibility gene for moyamoya disease, inhibits endoplasmic reticulumstress through SEL1L upregulation | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | RNF213 | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Endoplasmic reticulum stress | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | SEL1L | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | HRD1 | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Moyamoya disease | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
AHMED, SHARIF
× AHMED, SHARIF
|
|||||||
| 著者（ヨミ） | ||||||||
| 姓名 | アハメド, シャリフ | |||||||
| 言語 | ja | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | RNF213, a susceptibility gene for moyamoya disease, is associated with stress responses to various stressors. We previously reported that Rnf213 knockout (KO) mitigated endoplasmic reticulum (ER) stress-induced diabetes in the Akita mouse model of diabetes. However, the role of RNF213 in ER stress regulation remains unknown. In the present study, RNF213 knockdown significantly inhibited the upregulation of ER stress markers (CHOP and spliced XBP1) by chemical ER stress-inducers in HeLa cells. Levels of SEL1L, a critical molecule in ER-associated degradation (ERAD), were increased by RNF213 knockdown, and SEL1L knockdown prevented the inhibitory effect of RNF213 suppression on ER stress in HeLa cells, indicating SEL1L involvement in this inhibition of ER stress. SEL1L upregulation was also confirmed in pancreatic islets of Rnf213 KO/Akita mice and in Rnf213 KO mouse embryonic fibroblasts. Additionally, RNF213 suppression increased levels of HRD1, which forms a complex with SEL1L to degrade misfolded protein in cells under ER stress. In conclusion, we demonstrate that RNF213 depletion inhibits ER stress possibly through elevation of the SEL1L-HRD1 complex, thereby promoting ERAD in vitro and in vivo. | |||||||
| 言語 | en | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 本文/Department of Environmental and Molecular Medicine, Mie University Graduate School of Medicine, Edobashi 2-174, Tsu, Mie, 514-8507, Japan | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 7p | |||||||
| 書誌情報 |
発行日 2022-09-21 |
|||||||
| DOI | ||||||||
| 識別子タイプ | DOI | |||||||
| 関連識別子 | 10.1016/j.bbrc.2022.04.007 | |||||||
| フォーマット | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | application/pdf | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| 出版者 | ||||||||
| 出版者 | 三重大学 | |||||||
| 出版者（ヨミ） | ||||||||
| 値 | ミエダイガク | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(医学) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 14101 | |||||||
| 学位授与機関名 | 三重大学 | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2022-09-21 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲医学第2150号 | |||||||
| ノート | ||||||||
| 値 | Biochemical and Biophysical Research Communications 609 (2022) 62-68に掲載 | |||||||
| 資源タイプ（三重大） | ||||||||
| 値 | Doctoral Dissertation / 博士論文 | |||||||
