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miRNA-200c-3p targets talin-1 to regulate integrin-mediated cell adhesion
http://hdl.handle.net/10076/00021053
http://hdl.handle.net/10076/00021053f60a86cc-bb52-4f41-bde5-affc8039efc0
名前 / ファイル | ライセンス | アクション |
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2022DM0910 (2.4 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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公開日 | 2023-03-28 | |||||||
タイトル | ||||||||
タイトル | miRNA-200c-3p targets talin-1 to regulate integrin-mediated cell adhesion | |||||||
言語 | en | |||||||
言語 | ||||||||
言語 | eng | |||||||
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資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
資源タイプ | doctoral thesis | |||||||
アクセス権 | ||||||||
アクセス権 | open access | |||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
著者 |
OBENG, GIDEON
× OBENG, GIDEON
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著者(ヨミ) | ||||||||
姓名 | オベン, ギデオン | |||||||
言語 | ja | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | The ability of integrins on the cell surface to mediate cell adhesion to the extracellular matrix ligands is regulated by intracellular signaling cascades. During this signaling process, the talin (TLN) recruited to integrin cytoplasmic tails plays the critical role of the major adaptor protein to trigger integrin activation. Thus, intracellular levels of TLN are thought to determine integrin-mediated cellular functions. However, the epigenetic regulation of TLN expression and consequent modulation of integrin activation remain to be elucidated. Bioinformatics analysis led us to consider miR-200c-3p as a TLN1-targeting miRNA. To test this, we have generated miR-200c-3p-overexpressing and miR-200c-3p-underexpressing cell lines, including HEK293T, HCT116, and LNCaP cells. Overexpression of miR-200c-3p resulted in a remarkable decrease in the expression of TLN1, which was associated with the suppression of integrin-mediated cell adhesion to fibronectin. In contrast, the reduction in endogenous miR-200c-3p levels led to increased expression of TLN1 and enhanced cell adhesion to fibronectin and focal adhesion plaques formation. Moreover, miR-200c-3p was found to target TLN1 by binding to its 3-untranslated region (UTR). Taken together, our data indicate that miR-200c-3p contributes to the regulation of integrin activation and cell adhesion via the targeting of TLN1. | |||||||
言語 | en | |||||||
内容記述 | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | 本文/Department of Molecular Pathobiology and Cell Adhesion Biology, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan | |||||||
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内容記述タイプ | Other | |||||||
内容記述 | 21p | |||||||
書誌情報 |
発行日 2022-09-21 |
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DOI | ||||||||
識別子タイプ | DOI | |||||||
関連識別子 | 10.1038/s41598-021-01143-3 | |||||||
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内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
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出版タイプ | VoR | |||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
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出版者 | 三重大学 | |||||||
出版者(ヨミ) | ||||||||
値 | ミエダイガク | |||||||
学位名 | ||||||||
学位名 | 博士(医学) | |||||||
学位授与機関 | ||||||||
学位授与機関識別子Scheme | kakenhi | |||||||
学位授与機関識別子 | 14101 | |||||||
学位授与機関名 | 三重大学 | |||||||
学位授与年月日 | ||||||||
学位授与年月日 | 2022-09-21 | |||||||
学位授与番号 | ||||||||
学位授与番号 | 甲医学第2151号 | |||||||
ノート | ||||||||
値 | Scientific Reports (2021) 11:21597に掲載 | |||||||
資源タイプ(三重大) | ||||||||
値 | Doctoral Dissertation / 博士論文 |