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コイにおけるEdwardsiellatardaに対する免疫反応-II : 生菌ワクチン投与による能動免疫効果に関与する好中球の電子顕微鏡観察
http://hdl.handle.net/10076/13860
http://hdl.handle.net/10076/138603812f45c-a8cd-4f00-87a0-63d907129288
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
50C16965.pdf (8.4 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-04-28 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | コイにおけるEdwardsiellatardaに対する免疫反応-II : 生菌ワクチン投与による能動免疫効果に関与する好中球の電子顕微鏡観察 | |||||||||||||
| 言語 | ja | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | jpn | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | Edwardsiella tarda | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | active immunization | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | live bacteria of weakened-virulence | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | neutrophils | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | phagocytosis | |||||||||||||
| キーワード | ||||||||||||||
| 主題Scheme | Other | |||||||||||||
| 主題 | electron microscopy | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||
| 著者 |
島田, 真央
× 島田, 真央
× 宮﨑, 照雄
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| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | We studied on responses of neutrophils to E.tarda after an injection with the weakened-virulent bacteria and high-virulent bacteria with in the swimbladder in koi Cyprinus carpio, which were either unvaccinated or vaccinated by intramuscular injections with live bacteria of weakened-virulence. In the unvaccinated group challenge with the weakened-virulent bacteria（n=30）, infiltrated neutrophils phagocytized bacteria at 17-30 hs post challenge, and bacteria-laden neutrophils were decreased whereas normal cells were markedly increased at 48 hs. Electron microscopy（EM）revealed phagocytized bacteria were markedly damaged in the cell wall and cytoplasm with in the phagosomes, and formed secondary lysosomes in the cytoplasm. In the unvaccinated group challenged with the high-virulent bacteria（n=30）, neutrophils phagocytized bacteria at 17-30 hs, resulting in anincrease in fragmented cells at 48 hs. EM revealed neutrophils allowing propagation of undamaged bacteria in the cell. On the other hand in the vaccinated group challenge with the weakened-virulent bacteria（n=30）, many neutrophils phagocytized bacteria at 17-30 hs, and bacteria-laden neutrophils were decreased at 48hs. EM revealed phagocytized bacteria were markedly damaged with in the phagosomes and the cytoplasm of neutrophils. In another vaccinated group challenged with the high-virulent bacteria（n=30）, bacteria-laden neutrophils were increased between 30 and 48 hs whereas fragmented cells were markedly increased in just one fish in 48 hs. EM revealed phagocytized bacteria were markedly damaged within the phagosomes and the cytoplasm of neutrophils except for one fish that allowed bacterial propagation within neutrophils. These results determined that neutrophils were activated by immunization with live bacteria and successfully treated E. tarda of high-virulence as well as weak-virulence. | |||||||||||||
| 書誌情報 |
三重大学大学院生物資源学研究科紀要 巻 40, p. 7-20, 発行日 2014-03-01 |
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| 書誌レコードID | ||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||
| 収録物識別子 | AA12234310 | |||||||||||||
| フォーマット | ||||||||||||||
| 内容記述タイプ | Other | |||||||||||||
| 内容記述 | application/pdf | |||||||||||||
| 著者版フラグ | ||||||||||||||
| 出版タイプ | VoR | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||
| その他のタイトル | ||||||||||||||
| 言語 | en | |||||||||||||
| 値 | Immune Responses to Edwardsiella tarda in Koi-II : Electron Microscopic Studies on Nutrophils in Koi Immunizaed with Live Bacteria of E.tarda | |||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | 三重大学大学院生物資源学研究科 | |||||||||||||
| 資源タイプ（三重大） | ||||||||||||||
| 値 | Departmental Bulletin Paper / 紀要論文 | |||||||||||||
