| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2007-12-07 |
| タイトル |
|
|
タイトル |
Phenotypic complementation establishes requirements for specific POU domain and generic transactivation function of Oct-3/4 in embryonic stem cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Niwa, Hitoshi
Masui, Shinji
Chambers, Ian
Smith, Austin G.
Miyazaki, Jun-ichi
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Transcription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The POU factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal of pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, and A. G. Smith, Nat. Genet. 24:372-376, 2000). Here we have developed a complementation assay based on the ability of Oct-3/4 transgenes to rescue self-renewal in conditionally null ES cells and used this to define which domains of Oct-3/4 are required to sustain the undifferentiated stem cell phenotype. Surprisingly, we found that molecules lacking either the N-terminal or C-terminal transactivation domain, though not both, can effectively replace full-length Oct-3/4. Furthermore, a fusion of the heterologous transactivation domain of Oct-2 to the Oct-3/4 POU domain can also sustain self-renewal. Thus, the unique function of Oct-3/4 in ES cell propagation resides in combination of the specific POU domain with a generic proline-rich transactivation domain. Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation. |
| 内容記述 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
抗酸化系遺伝子改変動物を用いた母体糖尿病による胎児異常発生抑止効果に関する研究 |
| 書誌情報 |
Molecular and cellular biology
巻 22,
号 5,
p. 1526-1536,
発行日 2002-03-01
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0270-7306 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA10620925 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/MCB.22.5.1526-1536.2002 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| 出版者 |
|
|
出版者 |
American Society for Microbiology |
| シリーズ |
|
|
値 |
http://mcb.asm.org/cgi/reprint/22/5/1526 |
| ノート |
|
|
値 |
出版者版電子ジャーナルあり |
| 資源タイプ（三重大） |
|
|
値 |
Journal Article / 学術雑誌論文 |
30A1669.pdf (13.4 MB)
