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Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain
http://hdl.handle.net/10076/3116
http://hdl.handle.net/10076/3116d01be4c7-e712-43a9-9919-540741d8df9b
名前 / ファイル | ライセンス | アクション |
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AN100738460001504.pdf (391.2 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-05-10 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Simple Preparation of Large Amount of Lipopolysaccharide with Receptor Activity for Bacteriophage φXl74 from Escherichia coli C Strain | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | LPS | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | E..coli C strain | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | receptor | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | bacteriophage φX174 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
稲垣, 穣
× 稲垣, 穣× 加藤, 宗春× 大角, 善保× 貝谷, 康治× 西川, 司朗× 柏村, 直樹 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A convenient method for the preparation of a lipopolysaccharide (LPS) with receptor activity of bacteriophage φX174 from large amounts of the dried and defatted cells of Escherichia coli C strain was est.ablished. An improvement on bacterium cultivation led to yield over 75g of the dried and defatted cells (309g wet cells) from 6L culture medium containing 4% wt/v of D-glucose as a carbon source and 1 %wt/v of ammonium sulfate as a nitrogen source. The modified PCP method afforded 2.25g of LPS (3.0% yield from the cells) from 75g of the dried and defatted cells. It was confirmed that the obtained LPS showed in vitro an inactivation of bacteriophage φX174 from a plaque counting assay. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | バクテリオファージφⅩ174のレセプターとしての生理活性を有するリポ多糖を大腸菌C株の乾燥脱脂薗体から簡 便に大量調製する方法を確立した。培養条件を改良することによって炭素源としてグルコース4%wt/v,窒素源と して硫安1%wt/vを含む6Lの培養液から75gの乾燥脱脂菌体(湿菌体で309g)を得ることが出来た。得られた菌 体からPCP抽出改良法によってリポ多糖を抽出したところ,2.25gのリポ多糖が得られた(菌体重量の3.0%収率)。 得られたリポ多糖がバクテリオファージφⅩ174のレセプターとしての生理活性を有し,in vitroでのファージ不活 化能力を示すことがプラーク計数法で確認された。 |
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書誌情報 |
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 巻 15, p. 33-40, 発行日 1995-12-01 |
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ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0915-0471 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10073846 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 491 | |||||
その他のタイトル | ||||||
ja | ||||||
バクテリオファージφX174レセプター活性を有する大腸菌C株リポ多糖の簡便大量調製法 | ||||||
出版者 | ||||||
出版者 | Faculty of Bioresources, Mie University | |||||
ノート | ||||||
Agropedia提供データ | ||||||
資源タイプ(三重大) | ||||||
Departmental Bulletin Paper / 紀要論文 |