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Nafamostat mesylate prevents metastasis and dissemination of neuroblastoma through vascular endothelial growth factor inhibition
http://hdl.handle.net/10076/00021147
http://hdl.handle.net/10076/00021147a7b37026-7d47-442a-9443-173dee0059b7
名前 / ファイル | ライセンス | アクション |
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2022DM1220 (725.7 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||||
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公開日 | 2023-04-05 | |||||||||||
タイトル | ||||||||||||
タイトル | Nafamostat mesylate prevents metastasis and dissemination of neuroblastoma through vascular endothelial growth factor inhibition | |||||||||||
言語 | en | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
キーワード | ||||||||||||
主題Scheme | Other | |||||||||||
主題 | nafamostat mesylate | |||||||||||
キーワード | ||||||||||||
主題Scheme | Other | |||||||||||
主題 | neuroblastoma | |||||||||||
キーワード | ||||||||||||
主題Scheme | Other | |||||||||||
主題 | metastasis | |||||||||||
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主題Scheme | Other | |||||||||||
主題 | murine model | |||||||||||
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主題Scheme | Other | |||||||||||
主題 | vascular endothelial growth factor | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||||
資源タイプ | doctoral thesis | |||||||||||
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アクセス権 | open access | |||||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||||
著者 |
森本, 真理
× 森本, 真理
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抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Neuroblastoma is a highly malignant disease with a poor prognosis and few treatment options. Despite conventional chemotherapy for neuroblastoma, resistance, invasiveness, and metastatic mobility limit the treatment efficacy. Therefore, it is necessary to develop new strategies for treating neuroblastoma. The present study aimed to evaluate the anticancer effects of nafamostat mesylate, a previously known serine protease inhibitor, on neuroblastoma cells. Effects of nafamostat mesylate on neuroblastoma cell migration and proliferation were analyzed by wound healing assay and WST‑8 assay, respectively. To elucidate the mechanisms underlying the effects of nafamostat mesylate on neuroblastoma, the expression levels of NF‑κB were measured via western blotting, and the production of the cytokine vascular endothelial growth factor (VEGF) in the cell culture supernatants was determined via ELISA. In addition, a mouse model of hematogenous metastasis was used to investigate the effects of nafamostat mesylate on neuroblastoma. It was determined that nafamostat mesylate significantly inhibited migration and invasion of Neuro‑2a cells, but it had no effect on cell proliferationat 24 h after treatment. Exposure of Neuro‑2a cells to nafamostat mesylate resulted in decreased vascular endothelial growth factor production, which could be a pivotal mechanism underlying the inhibitory effects of neuroblastoma metastasis. The results of the present study suggest that nafamostat mesylate may be an effective treatment against neuroblastoma invasion and metastasis. | |||||||||||
言語 | en | |||||||||||
内容記述 | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | 本文/Department of Pediatrics, Mie University Graduate School of Medicine, Tsu, Mie 514‑8507, Japan | |||||||||||
内容記述 | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | 6p | |||||||||||
書誌情報 |
発行日 2022-12-21 |
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DOI | ||||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.3892/mco.2022.2571 | |||||||||||
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内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
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出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
出版者 | ||||||||||||
出版者 | 三重大学 | |||||||||||
出版者(ヨミ) | ||||||||||||
値 | ミエダイガク | |||||||||||
学位名 | ||||||||||||
学位名 | 博士(医学) | |||||||||||
学位授与機関 | ||||||||||||
学位授与機関識別子Scheme | kakenhi | |||||||||||
学位授与機関識別子 | 14101 | |||||||||||
学位授与機関名 | 三重大学 | |||||||||||
学位授与年月日 | ||||||||||||
学位授与年月日 | 2022-12-21 | |||||||||||
学位授与番号 | ||||||||||||
学位授与番号 | 乙医学第1081号 | |||||||||||
ノート | ||||||||||||
値 | MOLECULAR AND CLINICAL ONCOLOGY 17: 138, 2022に掲載 | |||||||||||
資源タイプ(三重大) | ||||||||||||
値 | Doctoral Dissertation / 博士論文 |