Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis

Isono,Naoto; Mizutani,Emi; Hayashida,Haruka; Katsuzaki,Hirotaka; Saburi,Wataru

doi:10.1016/j.bbrc.2022.07.098

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Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis

http://hdl.handle.net/10076/0002000013

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2023JA0004.pdf (1.6 MB)

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学術雑誌論文 / Journal Article(1)

公開日

2023-08-18

タイトル

Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis

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eng

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http://purl.org/coar/resource_type/c_6501

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journal article

著者

Isono,Naoto
Mizutani,Emi
Hayashida,Haruka
Katsuzaki,Hirotaka
Saburi,Wataru

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内容記述タイプ

Abstract

内容記述

Glycoside hydrolase family 94 (GH94) contains enzymes that reversibly catalyze the phosphorolysis of β-glycosides. We conducted this study to investigate a GH94 protein (PBOR_13355) encoded in the genome of Paenibacillus borealis DSM 13188 with low sequence identity to known phosphorylases. Screening of acceptor substrates for reverse phosphorolysis in the presence of α-D-glucose 1-phosphate as a donor substrate showed that PBOR_13355 utilized D-glucuronic acid and p-nitrophenyl β-D-glucuronide as acceptors. In the reaction with D-glucuronic acid, 3-O-β-D-glucopyranosyl-D-glucuronic acid was synthesized. PBOR_13355 showed a higher apparent catalytic efficiency to p-nitrophenyl β-D-glucuronide than to D-glucuronic acid, and thus, PBOR_13355 was concluded to be a novel glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase. PBOR_13360, encoded by the gene immediately downstream of the PBOR_13355 gene, was shown to be β-glucuronidase. Collectively, PBOR_13355 and PBOR_13360 are predicted to work together in the cytosol to metabolize oligosaccharides containing the 3-O-β-D-glucopyranosyl-β-D-glucuronide structure released from bacterial and plant acidic carbohydrates.

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en : Biochemical and Biophysical Research Communications

巻 625, p. 60-65, 発行日 2022-10-15

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収録物識別子タイプ

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収録物識別子

1090-2104

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2023-08-18 04:41:34.323935

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Functional characterization of a novel GH94 glycoside phosphorylase, 3-O-β-D-glucopyranosyl β-D-glucuronide phosphorylase, and implication of the metabolic pathway of acidic carbohydrates in Paenibacillus borealis

× Isono,Naoto

× Mizutani,Emi

× Hayashida,Haruka

× Katsuzaki,Hirotaka

× Saburi,Wataru

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