| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2007-07-02 |
| タイトル |
|
|
タイトル |
Cloning and Sequencing of Double-Stranded RNA |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
reverse transcription |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
polymerase chain reaction |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
double-stranded RNA |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
polyhedrin gene |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
point-mutation |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Bombyx mori cytoplasmic polyhedrosis virus |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
冨田, 昌弘
宮嶌, 成壽
吉村, 哲郎
鈴木, 義昭
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
All creatures, including procaryotes, harbor genetic information in deoxyribonucleic acid (DNA). Some viruses, however, store their genes in the form of ribonucleic acid (RNA) instead of DNA. To elucidate the functions of RNA viruses, determination of the nucleotide sequences making up their genetic material is necessary. However, direct sequencing of RNA is at present impossible. To address this problem, synthesis of complementary DNA (cDNA) using reverse transcriptase is generally applied. The problem is that the efficiency of this reverse reaction is extremely low. We have therefore employed the polymerase chain reaction to amplify cDNA after reverse transcription, and could thereby successfully determine the complete nucleotide sequence of a polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus, which harbors its genome as double-stranded RNA. |
| 書誌情報 |
Research reports of the Faculty of Engineering, Mie University
巻 22,
p. 69-77,
発行日 1997-12-24
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0385-6208 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA00816341 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| その他のタイトル |
|
|
言語 |
ja |
|
値 |
2本鎖RNA遺伝子のクローニングと塩基配列決定 |
| 出版者 |
|
|
出版者 |
Faculty of Engineering, Mie University |
| 資源タイプ（三重大） |
|
|
値 |
Departmental Bulletin Paper / 紀要論文 |
AA008163410220008.PDF (458.0 kB)
