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酵母細胞壁溶解酵素生産菌の分離とその同定
http://hdl.handle.net/10076/3252
http://hdl.handle.net/10076/3252b1d7820a-9d57-487f-befa-09000135873c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN100738460000511.pdf (449.6 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||||||||||
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| 公開日 | 2007-05-23 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | 酵母細胞壁溶解酵素生産菌の分離とその同定 | |||||||||||||||||||||
| 言語 | ja | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | jpn | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | yeast | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | lytic enzyme | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Fungi | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||||||||||
| 著者 |
山田, 哲也
× 山田, 哲也
× 西田, 淑男
× 久松, 眞
× 赤木, 盛郎
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | Eighty-two microorganisms lysing a yeast, Rhodosporidium sp., were separated from soil by the clear zone method. The media containing polypepton and aspartic acid, as the nitrogen source, and cell wall of Rhodosporidium sp. J-5B, as the carbon source, were used for the separation. A strain of Actinomycetes, No.39-B was selected from the isolated microbes for its good growth and lytic activity. When the No.39-B was cultivated in the medium contining 0.5% cell wall of Rhodosporidium and 0.05% glucose as the carbon source, it showed high lytic activity (10 units/ml) on hour 48. This enzyme was proven to be an induced type, because no activity was observed in the culture broth when the carbon source was changed from the cell wall to glucose. The crude enzyme was obtained from the culture broth by salting-out with 80% saturation of ammonium sulfate, dialysis, and lyophilization. The enzyme had lytic activities (degree of reduction in OD of living yeast suspension after incubation with 0.3% enzyme for 3 hr at 30℃) for yeasts; 68% to Rhodosporidium sp. J-5B 48% to Hansenula anomala, 38% to Saccharomyces diastaticus; 8% to Candida utilis, and 0% to Mycotorula japonica and Rhodotorula sa. Z-13. The strain of No. 39-B was estimated to be Actinomadura spadix from the data of morphological observation and chemotaxonomical tests showing existence of 2,6 diamino primeric acid and madurose. |
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| 書誌情報 |
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 巻 5, p. 133-139, 発行日 1991-01-31 |
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| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||||||||||||
| 収録物識別子 | 0915-0471 | |||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AN10073846 | |||||||||||||||||||||
| フォーマット | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | application/pdf | |||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||||
| 日本十進分類法 | ||||||||||||||||||||||
| 主題Scheme | NDC | |||||||||||||||||||||
| 主題 | 498 | |||||||||||||||||||||
| その他のタイトル | ||||||||||||||||||||||
| 言語 | en | |||||||||||||||||||||
| 値 | Study on Yeast Lytic Enzyme from Fungi | |||||||||||||||||||||
| 出版者 | ||||||||||||||||||||||
| 出版者 | 三重大学生物資源学部 | |||||||||||||||||||||
| ノート | ||||||||||||||||||||||
| 値 | Agropedia提供データ | |||||||||||||||||||||
| 資源タイプ（三重大） | ||||||||||||||||||||||
| 値 | Departmental Bulletin Paper / 紀要論文 | |||||||||||||||||||||
