| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2008-03-03 |
| タイトル |
|
|
タイトル |
酸化ストレスによるクラスターDNA損傷機構とその変異における役割(日本環境変異原学会第32回大会シンポジウム2「DNA損傷と変異のメカニズム」) |
|
言語 |
ja |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
reactive oxygen species |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
DNA damage |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
double base lesions |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
carcinogenesis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
hotspot |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
及川, 伸二
大西, 志保
村田, 真理子
平工, 雄介
川西, 正祐
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Reactive oxygen species generated by environmental factors, such as radiation, UV and chemicals can cause sequence-specific DNA damage and play important roles in mutagenesis and carcinogenesis. We have investigated sequence specificity of oxidative stress-mediated DNA damage by using ^<32>P-labeled DNA fragments obtained from the human c-Ha-ras-1, p53 and p16 genes. Free hydroxyl radicals cause DNA damage with no marked site specificity. Copper-hydroperoxo complex caused DNA damage at thymine, cytosine and guanine residues. ^1O_2 preferentially induces lesions at guanine residues. Benzoyloxyl radical specifically causes damage to the 5'-G in GG sequence ; this sequence is easily oxidized because a large part of the highest occupied molecular orbital of this radical is distributed on this site. Recently, we demonstrated that BP-7, 8-dione, a metabolite of carcinogenic benzo [a] pyrene (BP), strongly damaged the G and C of the 5'-ACG-3' sequence complementary to codon 273 of the p53 gene in the presence of NADH and Cu (II). BP-7, 8-dione also caused preferential double base lesion at 5'-TG-3' sequences. Since clustered DNA damage is poorly repaired, it is speculated that induction of the double base lesions in DNA might lead to activation of proto-oncogene or inactivation of the tumor suppressor gene. Therefore, oxidative DNA damage induced by BP-7,8-dione, especially double base lesions, may participate in the expression of carcinogenicity of BP in addition to DNA adduct formation. Here, we discuss the mechanisms of sequence-specific DNA damage including clustered DNA damage in relation to mutagenesis and carcinogenesis. |
| 書誌情報 |
環境変異原研究
巻 26,
号 2,
p. 125-133,
発行日 2004-09-30
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0910-0865 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AN1006217X |
| 権利 |
|
|
権利情報Resource |
本文データは学協会の許諾に基づきCiNiiから複製したものである |
|
権利情報 |
日本環境変異原学会 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 日本十進分類法 |
|
|
主題Scheme |
NDC |
|
主題 |
490 |
| その他のタイトル |
|
|
値 |
Mechanism of clustered DNA damage induced by oxidative stress and its role in mutagenesis and carcinogenesis(the symposium 2 "Mechanism of DNA damege and mutation" at the 32nd JEMS annual meeting, 2003.) |
| 出版者 |
|
|
出版者 |
日本環境変異原学会 |
| シリーズ |
|
|
値 |
http://ci.nii.ac.jp/naid/110001708215/ |
| 資源タイプ（三重大） |
|
|
値 |
Journal Article / 学術雑誌論文 |
30A6626.pdf (837.8 kB)
