胃癌細胞株ならびに胃間質細胞株の培養上清液より、別記の方法でエクソソームRNAを抽出しmicroRNA arrayに外注依頼し、データ解析し胃癌細胞株で異常発現している12miRNAを候補miRNAとして選定した。Preliminay studyとしれ胃癌患者(n=10)ならびに健常者(n=10)の血清よりエクソソームRNAを抽出しqRT-PCR法で候補miRNAを定量し、統計解析ソフトでリスクスコアを作成した所、胃癌患者では健常人と比べてリスクスコアが有意に高値であり(P<0.0001)、リスクスコアによってArea Under Curve(AUC):0.966の精度で胃癌患者を識別しえた。
We extracted exosomal-RNA from the culture-supernatant of gastric cancer cell line and gastric stromal cell line by the method mentioned separately, offered microRNA-array to outsource, then analyzed the data and identified 12 miRNA which were significantly dysregulated in gastric cancer cell line. As a preliminay study, exosomal RNA was extracted from the sera of gastric cancer patients (n=10) and healthy subjects(n=10). Then the candidate miRNA were quantified by qRT-PCR method, and risk score was created by statistical software. The risk score was significantly higher in gastric cancer patients than that in healthy controls (p<0.0001), and discriminated gastric cancer patients by the accuracy pf Area Under Curve (AUC): 0.966.
エクソソームに包埋された核酸を用いた胃癌早期診断法の確立
2021RP0033
(193.21KB)
