{"_buckets": {"deposit": "0364054c-1ebb-4ab3-9aa2-2e26e39e704d"}, "_deposit": {"created_by": 15, "id": "14499", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "14499"}, "status": "published"}, "_oai": {"id": "oai:mie-u.repo.nii.ac.jp:00014499", "sets": ["1692670486782"]}, "author_link": [], "item_8_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-05-18", "bibliographicIssueDateType": "Issued"}}]}, "item_8_description_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_8_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "胃癌細胞株ならびに胃間質細胞株の培養上清液より、別記の方法でエクソソームRNAを抽出しmicroRNA arrayに外注依頼し、データ解析し胃癌細胞株で異常発現している12miRNAを候補miRNAとして選定した。Preliminay studyとしれ胃癌患者(n=10)ならびに健常者(n=10)の血清よりエクソソームRNAを抽出しqRT-PCR法で候補miRNAを定量し、統計解析ソフトでリスクスコアを作成した所、胃癌患者では健常人と比べてリスクスコアが有意に高値であり(P\u003c0.0001)、リスクスコアによってArea Under Curve(AUC):0.966の精度で胃癌患者を識別しえた。", "subitem_description_type": "Abstract"}, {"subitem_description": " We extracted exosomal-RNA from the culture-supernatant of gastric cancer cell line and gastric stromal cell line by the method mentioned separately, offered microRNA-array to outsource, then analyzed the data and identified 12 miRNA which were significantly dysregulated in gastric cancer cell line. As a preliminay study, exosomal RNA was extracted from the sera of gastric cancer patients (n=10) and healthy subjects(n=10). Then the candidate miRNA were quantified by qRT-PCR method, and risk score was created by statistical software. The risk score was significantly higher in gastric cancer patients than that in healthy controls (p\u003c0.0001), and discriminated gastric cancer patients by the accuracy pf Area Under Curve (AUC): 0.966.", "subitem_description_type": "Abstract"}]}, "item_8_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "2017年度~2019年度科学研究費補助金(基盤研究(C))研究成果報告書", "subitem_description_type": "Other"}]}, "item_8_description_64": {"attribute_name": "科研費番号", "attribute_value_mlt": [{"subitem_description": "17K10583", "subitem_description_type": "Other"}]}, "item_8_publisher_30": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "三重大学"}]}, "item_8_text_31": {"attribute_name": "出版者(ヨミ)", "attribute_value_mlt": [{"subitem_text_value": "ミエダイガク"}]}, "item_8_text_65": {"attribute_name": "資源タイプ(三重大)", "attribute_value_mlt": [{"subitem_text_value": "Kaken / 科研費報告書"}]}, "item_8_version_type_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "吉山, 繁幸", "creatorNameLang": "ja"}, {"creatorName": "ヨシヤマ, シゲユキ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Yoshiyama, Shigeyuki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "問山, 裕二", "creatorNameLang": "ja"}, {"creatorName": "トイヤマ, ユウジ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Toiyama, Yuji", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "楠, 正人", "creatorNameLang": "ja"}, {"creatorName": "クスノキ, マサト", "creatorNameLang": "ja-Kana"}, {"creatorName": "Kusunoki, Masato", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "安田, 裕美", "creatorNameLang": "ja"}, {"creatorName": "ヤスダ, ヒロミ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Yasuda, Hiromi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "毛利, 靖彦", "creatorNameLang": "ja"}, {"creatorName": "モウリ, ヤスヒコ", "creatorNameLang": "ja-Kana"}, {"creatorName": "Mohri, Yasuhiko", "creatorNameLang": "en"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2022-01-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2021RP0033.pdf", "filesize": [{"value": "193.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 193200.0, "url": {"label": "2021RP0033", "url": "https://mie-u.repo.nii.ac.jp/record/14499/files/2021RP0033.pdf"}, "version_id": "bed77a13-cfe9-4ed6-aef8-2a2e330d1d22"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "胃癌", "subitem_subject_scheme": "Other"}, {"subitem_subject": "バイオマーカー", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "エクソソームに包埋された核酸を用いた胃癌早期診断法の確立", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "エクソソームに包埋された核酸を用いた胃癌早期診断法の確立", "subitem_title_language": "ja"}, {"subitem_title": "establishment of early diagnostic method in gastric cancer using exosome RNA", "subitem_title_language": "en"}]}, "item_type_id": "8", "owner": "15", "path": ["1692670486782"], "permalink_uri": "http://hdl.handle.net/10076/00020117", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2022-01-19"}, "publish_date": "2022-01-19", "publish_status": "0", "recid": "14499", "relation": {}, "relation_version_is_last": true, "title": ["エクソソームに包埋された核酸を用いた胃癌早期診断法の確立"], "weko_shared_id": -1}
エクソソームに包埋された核酸を用いた胃癌早期診断法の確立
http://hdl.handle.net/10076/00020117
http://hdl.handle.net/10076/00020117