| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2011-12-15 |
| タイトル |
|
|
タイトル |
In vivo time-course imaging of tumor angiogenesis in colorectal liver metastases in the same living mice using two-photon laser scanning microscopy |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Tanaka, Koji
Morimoto, Yuhki
Toiyama, Yuji
Matsushita, Kohei
Kawamura, Mikio
Koike, Yuhki
Okugawa, Yoshinaga
Inoue, Yasuhiro
Uchida, Keiichi
Araki, Toshimitsu
Mizoguchi, Akira
Kusunoki, Masato
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
In vivo real-time visualization of the process of angiogenesis in secondary tumors in the same living animals presents a major
challenge in metastasis research.We developed a technique for intravital imaging of colorectal liver metastasis development in live
mice using two-photon laser scanning microscopy (TPLSM). We also developed time-series TPLSM in which intravital TPLSM
procedures were performed several times over periods of days to months. Red fluorescent protein-expressing colorectal cancer cells
were inoculated into the spleens of green fluorescent protein-expressing mice. First- and second-round intravital TPLSM allowed
visualization of viable cancer cells (red) in hepatic sinusoids or the space of Disse. Third-round intravital TPLSM demonstrated
liver metastatic colonies consisting of viable cancer cells and surrounding stroma with tumor vessels (green). In vivo time-course
imaging of tumor angiogenesis in the same living mice using time-series TPLSM could be an ideal tool for antiangiogenic drug
evaluation, reducing the effects of interindividual variation. |
| 書誌情報 |
Journal of oncology
巻 2012,
p. 265487,
発行日 2011-11-03
|
| ISSN |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1687-8469 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 |
|
|
出版者 |
Hindawi Publishing Corporation |
| 資源タイプ（三重大） |
|
|
値 |
Journal Article / 学術雑誌論文 |
39A15352.pdf (4.9 MB)
