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堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質
http://hdl.handle.net/10076/3043
http://hdl.handle.net/10076/3043bc0a2028-9bb6-41f1-b983-0cc22ce4a0c8
名前 / ファイル | ライセンス | アクション |
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-04-26 | |||||
タイトル | ||||||
タイトル | 堆肥から分離したClostridium thermosaccharolyticum F - 9の β - グルコシダーゼ の性質 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thermophilic | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | β-glucosidase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | thermostable enzyme | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
嶋田, 協
× 嶋田, 協× 袴田, 佳宏× 粟冠, 和郎× 松嶋, 欽一 |
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著者別名 |
Shimada, Kyo
× Shimada, Kyo× Hakamada, Yoshihiro× Sakka, Kazuo× Matsushima, Kinichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Nine strains of cellulolytic-thermophilic anaerobes were isolated from hot springs and composts and analyzed for their productivity of β-glucosidase (β-D-Glucoside glucohydrolase EC 3. 2. 1. 21). Among them, strain F-9 showed the highest β-glucosidase activity, and this strain was identified as a member of Clostridium thermosaccarolyticum on taxonomical characteristics. The intracellular β-glucosidase of the bacterium was purified 40-fold with a recovery of 6%. The purified enzyme preparation was chromatographically homogeneous. The molecular weight of about 350,000 was estimated by gel filtration using Sephadex G-150, Bio-Gel p-300 and Sepharose 4B. Its isoelectric point was estimated to be 4.6. The activity of the enzyme for cellobiose was very much lower than for p-nitrophenyl β-D-glucoside. The enzyme did not hydrolyse maltose, lactose, carboxy- methyl-cellulose and cellulose. It was strongly inhibited by D-glucono-δ-lactone and was sensitive to thiol reagents. |
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書誌情報 |
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 巻 2, p. 81-84, 発行日 1989-03-30 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0915-0471 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN10073846 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 463 | |||||
その他のタイトル | ||||||
Some Properties of β‐Glucosidase from Clostridium thermosaccharolyticum F - 9 Isolated from Compost | ||||||
出版者 | ||||||
出版者 | 三重大学生物資源学部 | |||||
ノート | ||||||
Agropedia提供データ | ||||||
資源タイプ(三重大) | ||||||
Departmental Bulletin Paper / 紀要論文 |