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アイテム
ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討
http://hdl.handle.net/10076/3319
http://hdl.handle.net/10076/3319ce2ba723-47fd-4bfd-9633-3baf2f866db3
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN100738460000707.pdf (252.4 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2007-05-30 | |||||
| タイトル | ||||||
| タイトル | ＩＳＦＥＴバイオセンサシステムによるＬ－グルタミン定量の可能性の検討 | |||||
| 言語 | ja | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ISFET | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | biosensor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glutamine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | glutaminase | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | enzyme immobilization | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
田口, 寛
× 田口, 寛× 若林, 豊× 石原, 則幸× 奥村, 克純× 嶋林, 幸英 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A glutamine sensor was constructed by immobilizing L-glutaminase with glutaraldehyde on ISFET (ion selective field effect transistor) to investigate the possibility of determining L-glutamine. The prin- ciple of this sensor is as follows. The output voltage from ISFET, which depends on a very minute pH elevation due to the ammonium ion formation according to the enzymic reaction, is amplified with a differential amplifier of our own assembled. The amplified voltage was processed automatically with an on-line personal computer via GP-IB interface on a real-time basis. The calibration line for L-glutamine was linear from 1mM to 40mM and the relation coefficient was 0.967. The sensitivity of this system to L-glutamine was 0.5mM. The time needed for deter- mining one sample was within 13 min. The substrate specificity of this sensor was strict and L- glutamine was the sole compound responding to this sensor among analogues tested. D-glutamine was completely inactive to this sensor. It is suggested that L-glutanrine can be determined with this newly constructed ISFET-biosensor system with L-glutaminase. |
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| 書誌情報 |
三重大学生物資源学部紀要 = The bulletin of the Faculty of Bioresources, Mie University 巻 7, p. 71-75, 発行日 1992-01-31 |
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| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0915-0471 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN10073846 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 日本十進分類法 | ||||||
| 主題Scheme | NDC | |||||
| 主題 | 549 | |||||
| その他のタイトル | ||||||
| 言語 | en | |||||
| 値 | Investigation of Possibility of Determining L-Glutamine with the ISFET-Biosensor System | |||||
| 出版者 | ||||||
| 出版者 | 三重大学生物資源学部 | |||||
| ノート | ||||||
| 値 | Agropedia提供データ | |||||
| 資源タイプ（三重大） | ||||||
| 値 | Departmental Bulletin Paper / 紀要論文 | |||||
